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                产品中心-北京博奥森生物技术有限公司
                首页 > 产品中心 > 常用试剂 > 产品信息
                DAB kit (20×) (C-0010)
                ~~~促销,代码CY002~~~
                订购热线:400-901-9800
                订购邮箱:sales@bioss.com.cn
                订购传真:010-58129612
                技术支持:support@bioss.com.cn
                说明书: 下载
                0.5ml×2/70.00元
                1ml×2/120.00元
                大包装/询价
                产品编号 C-0010
                英文名称 DAB kit (20×)
                中文名称 DAB显色试剂盒(20×)
                别    名 DAB Substrate; DAB Horseradish Peroxidase Color Development Kit; DAB HRP Color Development Kit;   
                Specific References  (25)     |     C-0010 has been referenced in 25 publications.
                [IF=8.355] Chen Z et al. Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers. Chemical Engineering Journal,2019 375, 122109.   IHC ;  
                [IF=5.5] Yu JL et al. Lentivirus-Mediated Gene Silencing of Lymphocyte Function-Associated Antigen 1 Inhibits Apoptosis of Hippocampal Neurons in Rats with Acute Cerebral Ischemia After Cerebral Lymphatic Blockage.(2018) Cell Physiol Biochem.51(3):1069-1086.  IHC-P ;  
                [IF=5.391] Yang C et al. Long?noncoding?RNA?HAGLR?acts?as a?microRNA-143-5p?sponge?to?regulate?epithelial-mesenchymal?transition?and?metastatic?potential?in?esophageal?cancer?by regulating LAMP3. FASEB J. 2019 Jul 15:fj201802543RR.   IHC-P ;  
                [IF=5.047] Chen Z et al. Synergistic antitumor efficacy of hybrid micelles with mitochondrial targeting and stimuli-responsive drug release. Journal of Materials Chemistry B.2019.  IHC-P ;  -.  
                [IF=4.522] Ma S et al. Histone?deacetylases?inhibitor?MS-275?suppresses?human?esophageal?squamous?cell?carcinomacell?growth?and?progression?via?the?PI3K/Akt/mTOR?pathway. J Cell Physiol. 2019 May 23.   IHC-P ;  
                [IF=3.923] Gao ZF et al. microRNA-107 protects against inflammation and endoplasmic reticulum stress of vascular endothelial cells via KRT1-dependent Notch signaling pathway in a mouse model of coronary atherosclerosis.(2018) J Cell Physiol.  IHC-P ;  
                [IF=3.454] Sun DZ et al. Upregulated?MicroRNA-141?Suppresses?Epithelial-Mesenchymal?Transition?and?Lymph?NodeMetastasis?via?HOXC6-Dependent?TGF-β?Signaling?Pathway?in Laryngeal Cancer. Am J Physiol Cell Physiol. 2019 Apr 24.   IHC-P ;  
                [IF=3.454] Hong X et al. MicroRNA-150 Suppresses Epithelial Mesenchymal Transition, Invasion and Metastasis in Prostate Cancer through the TRPM4-mediated β-catenin Signaling Pathway.(2018) Am. J. Physiol., Cell Physiol.  IHC-P ;  
                [IF=3.448] Qu S et al. MicroRNA-194?reduces?inflammatory?response?and?human?dermal?microvascular?endothelialcells?permeability?through?suppression?of TGF-β/SMAD?pathway?by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem. 2019 Jun 12.  IHC ;  
                [IF=3.448] Gao J et al. Long noncoding LINC01551 promotes hepatocellular carcinoma cell proliferation, migration, and invasion by acting as a competing endogenous RNA of microRNA?… J Cell Biochem. 2019 Jul 3.   IHC ;  
                [IF=3.404] Xiao L et al. Development of a novel drug targeting delivery system for cervical cancer therapy.(2019) Nanotechnology. 30(7):075604.  ICC ;  
                [IF=3.266] Lin J et al. Ginsenoside Rb1?improves?learning and memory?ability?through its?anti-inflammatory?effect?in Aβ1-40?induced Alzheimer's disease of rats. Am J Transl Res. 2019 May 15;11(5):2955-2968.  IHC ;  
                [IF=3.12] Li X et al.Ginsenoside Rg3 Decreases NHE1 Expression via Inhibiting EGF-EGFR-ERK1/2-HIF-1 α Pathway in Hepatocellular Carcinoma: A Novel Antitumor Mechanism. (2018) Am. J. Chin. Med.46(8)  IHC-P ;  
                [IF=2.669] Jiang L et al. GYY4137 attenuates LPS-induced acute lung injury via heme oxygenase-1 modulation. Pulm Pharmacol Ther. 2019 Feb;54:77-86.  IHC ;  -.  
                [IF=1.682] Zhu ZY et al. Silencing of MEOX1 Gene Inhibits Proliferation and Promotes Apoptosis of LNCaP Cells in Prostate Cancer. (2018) Cancer Biother. Radiopharm.  IHC-P ;  
                [IF=1.654] Li T et al. Histomorphological Comparisons and Expression Patterns of BOLL Gene in Sheep Testes at Different Development Stages.Animals (Basel). 2019 Mar 21;9(3).   IHC ;  
                [IF=0] Wang Y et al. ANXA3 silencing ameliorates intracranial aneurysm via inhibition of the JNK signaling pathway. Molecular Therapy: Nucleic Acid.2019 JUN.  IHC ;  
                [IF=3.331] Wang X et al. Characterization of GLOD4 in Leydig Cells of Tibetan Sheep During Different Stages of Maturity.Genes (Basel). 2019 Oct 12;10(10). pii: E796.  IHC-P ;  
                [IF=] Liu P et al. Circular RNA-hsa-circ-0000670 promotes gastric cancer progression through the microRNA-384/SIX4 axis. Exp Cell Res . 2020 Sep 15;394(2):112141.  IHC-P ;  
                [IF=] Mei X et al. Expression of VEGF, CD73 and their relationship with clinical pathology, microvessel density, and prognosis in renal cell carcinoma. Transl Androl Urol. 2020 Jun;9(3):1366-1373.  IHC-P ;  
                [IF=] Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev . 2020 Jul 9;2020:6428498.  IHC ;  
                [IF=] Dai Caoet al. Luteolin suppresses epithelial-mesenchymal transition and migration of triple-negative breast cancer cells by inhibiting YAP/TAZ activity. Biomed Pharmacother . 2020 Sep;129:110462.  other ;  
                [IF=] Yi-Feng Yuanet al. Cyclopamine functions as a suppressor of benign prostatic hyperplasia by inhibiting epithelial and stromal cell proliferation via suppression of the Hedgehog signaling pathway. Int J Mol Med . 2020 Jul;46(1):311-319.  IHC ;  
                [IF=] Gaomei Lvet al. Silencing ICAM-1 reduces the adhesion of vascular endothelial cells in mice with immunologic contact urticaria. Gene . 2020 Nov 15;760:144965.  IHC ;  
                [IF=] Wang N et al. Dysregulated YY1/PRMT5 axis promotes the progression and metastasis of laryngeal cancer by targeting Hippo pathway  IHC ;  
                保存条件 Store at –20℃.
                产品介绍 DAB (3,3′-Diaminobenzidine; 3,3′,4,4′-Biphenyltetramine; 3,3′,4,4′-Tetraaminobiphenyl) is Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually. The kit contains three components: the DAB Chromogen, Substrate Buffer and Staining Assistant. All components are in liquid form. This new 3-Solution DAB product has improved stability, ease of formulation, and greater sensitivity than previous DAB products.

                Important Note:
                This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

                DAB,即3,3’-二氨基联苯胺(3,3’-diaminobenzidine)是辣根过氧化物酶(Peroxidase)的常用生色底物,在过氧化氢的存在下失去电子而呈现出颜色变化,形成浅棕色不溶性产物。用于检测过氧化物酶的活性,它灵敏度高,特异性好,是HRP结合物最常用的底物,常在免疫组化,原位杂交,Western?blot等膜显色或检测细胞或组织内源性的过氧化物酶中应用广泛。
                用法:
                1. 临用前配制 10 mL 1×DAB 显色工作液:将 0.5 mL 20× DAB 显色液 A 加入 9 mL 1×PBS 中并涡旋混匀。向 DAB / PBS 混合物中加入0.5 mL 20×DAB显色液B涡旋混合均匀。 可以根据需要按比例缩放体积。 注意:不要使用含有叠氮化钠的缓冲液,叠氮化钠是一种HRP抑制剂。
                2. 在样品的最后一次洗涤完毕后,去除洗涤液,加入足够 的 DAB 显色工作液,确保能充分覆盖样品。室温避光孵育 3-30 分钟或更长时间,直至显色达到预期深浅。免疫印迹实验,应保证样品在一个足够多的染色液中且能被自由晃动。
                3. 去除 DAB 染色工作液,用 PBS 冲洗样品几次以中止显色反应。对于用显微镜观察的细胞或组织样品,可复染样品(可选)并安装在水性封固介质中。或者,可以将样品脱水并安装在有机封固剂中。免疫印迹实验,可将样品用水冲洗,风干,然后在室温下储存。

                储存与运输:
                -20℃避光密闭保存,一年有效。避免反复冻融。
                2~8℃运输,在途时间5天对试剂无影响。

                注意事项
                1. DAB对人体有害,操作时请小心,并注意有效防护以避免直接接触人体或吸入体内。
                2. 显色工作液应现用现配,新鲜配制的工作液应为无色或浅棕色,如颜色过深,请勿使用。??
                3. 显色时间严格控制,根据情况调整,以免显色过度。固相膜显色数小时后即会褪色,不能永久存在。
                4. 请将沾有DAB显色液A的容器等放在含有3% KMnO4,2% NaHCO3?的溶液浸泡3h,以减少污染。?
                5. 本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
                6. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
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